Choose a good topic for this paper Essay Example | Topics and Well Written Essays - 500 words

Choose a good topic for this paper - Essay Example Quite rightly so, in order to find out how something is morally right or wrong, the need is to know where the line has to be drawn because then only can the distinctions be made between the right and wrong steps. If actions and undertakings bring in a positive aspect to the fore where there is no problem for the people concerned with it, then there is understanding available. This optimism shall suggest for further usage of ethics in a quantifiable manner. However, it is important to know that the flip side is equally fatal. The unethical means are also significant to draw and gain something from (Shafer-Landau, 2012). What this will do is to inform everyone how things shall shape up and how sanity needs to be restored. As a parent, this seems like a difficult process all along, yet the basis for comprehension is there for one and all to see. This oxymoron is apparent because ethics seem to differ from person to person. There are some individuals who believe that ethics is all about doing ‘good’ for one’s own self but not for others. This is a flawed mindset which has drastic results in the end, and more so for the societal regimes. Ethics when taught to the young ones should always be in such a manner that there are more positive undertakings for everyone irrespective of his position within the society. It needs to comprise of the collective goodwill of the society to bring forward seriously positive results. Essentially speaking and focused more towards the junior-high age youngsters, teaching ethics to them is a difficult process as they might have readymade alternatives for them. With so much negativity being highlighted by the media and the different domains of the society, the children can only be confused rather than getting corrected. In the end, it would be justifiable to say that there is a dire need on the part of the parents and teachers to enact ways and means to bring correction which is long-lasting and has a positive

Effect of cancer medical research on tobacco industry Case Study

Effect of cancer medical research on tobacco industry - Case Study Example One of the possible solutions to the industry is selling tobacco products to the young people and women. As noted by WHO (2008) early smokers start as early as the age of 10. Therefore as the tobacco industry faces a lot of challenges, they are compelled to majorly target the youths and women in the society. Teenagers are assumed to be the potential future consumers of tobacco because they are greatly impressionable and in most communities, they have the significant amount of money at their disposal (Mackay & Eriksen, 2002). For this reason, they are easily impressed by any company where the consumption and brand of products are good. Despite many of the tobacco industry’s products killing many customers and forcing others to quit, they make sure they have a new target to consume their products. This is why they go for the young people since they will take a longer span before dying or eventually quitting hence increasing chances of continued salesGenerally, women smoke less t han the potential smokers; men. It is dangerous to the unborn but still, tobacco industry considers women as the untapped group which if tapped can increase and improve its consumption than in men. Hence, unchecked and their greatest motive being profit making, tobacco industries in considerably targets the children and women whom they believe can increase the likelihood of continued sales. Recently, the danger imposed to people’s health by tobacco intake has been confirmed and since they knew this long time ago, but have been attempting not to disclose this research (Mackay & Eriksen, 2002). Other nations, for instance, US have always had the political will and resources to deal with the tobacco’s large corporations. However, rich countries that have smaller but tougher markets have made multinational firms dealing with tobacco to intensify their efforts in different regions especially Asia to keep up the growth and selling of cigarettes alongside expanding advertisement to increase a level of demand if not to meet it completely.

E-Business Foundation Essay Example | Topics and Well Written Essays - 750 words

E-Business Foundation - Essay Example The main redeeming aspect about Google’s approach has been its innovative approach.Thus, it is seen that all their website projects integrate the latest thinking on design, usage, accessibility, technology and marketing strategy. Google has been serving their customers for over a decade now. They have attributed their success and long life to their quest for making rewarding and long-term relationships with both potential and existing clients it is believed that Google take special interest in the success of their clients and work hand- in- hand to ensure that the product is of high quality that could meet the clients’ goals and objectives. Google has decided to take over YouTube for $1.65 million in stocks. This would spell great opportunities for internet users and content owners throughout the business world. The takeover merges one of the largest and fastest growing online video entertainment businesses with Google's proven proficiency in organizing information and creating new models for advertising on the Internet. The merged companies would focus on providing a better, more comprehensive experience for users interested in uploading, watching and sharing videos, and will offer new opportunities for professional content owners to distribute their work to reach vast new viewers. Next, Google has also received approval from the US Federal regulators to acquire Double Click for $3.1 billion. Google generates revenue from selling text-based ads that appear next to search results.

Economics Assessment Assignment Example | Topics and Well Written Essays - 1500 words - 1

Economics Assessment - Assignment Example For the purpose of this study we have used a product as a guinea pig. In order to understand the impact of the product in its related market and the impact of the market on the product, we have carried out further analysis using the established theories of economics about the future viability of the product. Here for the sake of this analysis we have used the potato chips product manufactured by the Coca Cola Company called LAYS. (Lipsey and Chrystal, 2002) The price elasticity of demand is defined as the magnitude of the proportionate change in demand and the proportionate change in price of the product. Therefore, elasticity is the measure of responsiveness. Price elasticity of demand is a very important concept that is related to the pricing decisions of a product. If an organization wants to generate the maximum amount of revenue from its products and it is unable to determine how much increase in the price can be made by them so that the overall demand of the product does not reduce. This decision is taken on the basis of the analysis of price elasticity of demand. The price elasticity of demand can be described as the rate of response of quantity demanded due to a price change. This means that if a unit change in the price of the produc

American college student Assignment Example | Topics and Well Written Essays - 1000 words

American college student - Assignment Example This is a psychosocial theory that is developed on four pillars that include situation, self, strategy and support (Isaacson, 2008). This psychosocial theory helps individuals be able to address issues in their lives since they aid people to determine what and how they would love to live their lives thereafter. Collaborating with other institutions around the college that provide services relevant to the courses that the students are undertaking is also vital in training them how to work in their respective fields. This can be coupled with Kuh’s collaborative assignments and projects. Kuh (2008) says that they help students learn how to work as well as sharpen their ability to grasp things and work while incorporating other people’s ideas in order to produce fine-tuned results. Kuh (2008) states that this is done through ways like â€Å"study groups, assignments done in groups, and projects and research work done cooperatively†. This also helps cement the place o f the college within the community and among the stakeholders if the students come out better people, thus; the college reputation remains good for a long time. Question 2 Creating a program that will enable students to achieve at least two of Kuh’s high-impact educational practices, it is imperative to include all the students. Programs aimed at a more tenured faculty with a better faculty student interaction should be encouraged (Kuh, 2008). This is so to ensure that all the students from different, but related faculties can share ideas and learn how to work in two or more related fields towards a common goal. This also enables students to interact more with their faculty professors in an attempt to understand their course work better. Additionally, students obtain a platform to ask questions that are helpful in their course work and in building their careers. This ensures and helps students irrespective of who they are to achieve the common intellectual experience of Kuhâ €™s high-impact educational practices (Kuh, 2008). Faculties should also encourage students to get more involved in research work and courses to learn more, instead of dwelling mainly on theory. Students should be able to test the authenticity of whatever they are taught in class in order to ascertain the credibility of information received and even to learn how to perform outdoor functions (Jones & Abes, 2013). Students doing their own research learn self-independence and the art of referencing. Other skills that come with conducting research are also gained by all the students. Sharing these ideas in class will, in effect, help them compare notes on what the most effective methods to use are and how to employ them. This line of approach actually helps achieve Kuh’s other emphasis which is involvement in undergraduate research (Kuh, 2008). Intensive and thorough internship programs with grades attached to the time and effort placed by the students during their placement is another way of ensuring that all the students are involved in learning. Their assessment during the internship is done by the idustrial supervisor who will ensure that they master how to do their delegated duties in perfection. Kuh states that internship provides students with the professional coaching; thus, they can benefit from real work environment experience (Kuh, 2008). Question 1 Chickering’

Bald Eagle, Great white shark and Siberian Tiger Essay Example for Free

Bald Eagle, Great white shark and Siberian Tiger Essay There are three types of animals around the world known to be endangered species. We can help and protect them by helping the people aware of this animal, so they will be able to know the importance of keeping the animals to live longer.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The Bald Eagle is not really bald; it actually has white feathers on its head, neck, and tail. Bald is a derivation of balde, and Old English word meaning white. The eagle was names for its white feathers instead for a lack of feathers. Bald eagles may use the same nest year after year, adding more twigs and braches each time. One nest found that had been used for 34 years and weighted over two tons. The bald eagle can fly 20 to 40 mph in normal flight and can dive at speeds over 100mph. Bald eagles can actually swim. They use an overhand movement of wings that is very much like the butterfly stroke. More than 80% of bald eagle population in the southeastern United States is concentrated within the state of Florida.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The great white shark is a streamlined swimmer and a ferocious predator with 3,000 teeth at any one time. This much-feared fish has a torpedo-shaped body, a pointed snout, a crescent-shaped tail, 5 gill slits, no fin spines, an anal fin, and 3 main fins: the dorsal fin (on its back) and 2 pectoral fins (on its sides). When the shark is near the surface, the dorsal fin and part of the tail are visible above the water.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The Siberian Tiger (also called the Amur Tiger) is the largest member of the cat family. These huge, territorial cats are found in the Amur-Ussuri region of Siberia (in northeastern Russia) and in northern China and Korea. They live in mountainous conifer forests at elevations up to 3,000 feet. There are only about 400 of these magnificent tigers left in the wild; they are an endangered species. Male Siberian tigers are up to 10 3/4 ft (3.3 m) long and weigh up to 660 pounds (300 kg); females are up to 8 1/2 ft (2.6 m) long and weigh up to 370 pounds (165 kg). The fur is usually pale orange-brown with black stripes; they are paler than most other tigers. The fur on the belly and chest is white with black stripes. There is a white ruff around the neck.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   In the United States, Bald Eagle is a symbol of justice, strength, and fairness. Great white shark Carcharodon carcharias, also known as white pointer, white shark, or white death, is an exceptionally large lamniform shark found in coastal surface waters in all major oceans. Siberian Tigers are the biggest cats in the world. They live in steamy hot jungles as well as icy cold forest habitats. There are five different kinds or subspecies of tiger alive in the world today.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Therefore, these are three animals are considered as the most endangered animals around the world. They also seek for love and care. As we people of god, our responsibility is to protect them out from danger.   Let them live with peace and harmony. References: Endangered Animals (1996) Bald Eagle. World Wide Web:   http://www.worldkids.net/eac/eagle.html 5k Enchanted Learning Software. Great White Shark. World Wide Web: http://enchantedlearning.com/subjects/sharks/species/Greatwhite.shtml 25k Enchanted Learning.com. Siberian Tiger. World Wide Web: http://www.enchantedlearning.com/subjects/mammals/tiger/Siberiatigerprintout.shtml 9k

Methods of DNA Identification

Methods of DNA Identification To isolate DNA from blood, saliva, buccal swab and betel quid by phenol-chloroform method and chelex method and compare the efficacy of both the methods. To carry out restriction digestion of the DNA samples isolated from above mentioned sources using the restriction enzyme EcoRI (G|AATTC) and identify individuals based on the pattern of restriction banding and to ascertain the applicability of the restriction digestion in forensics MATERIALS AND METHODS: Blood, saliva, betel quid and buccal swab were collected from 15 patients and DNA isolation was done by phenol-chloroform method and chelexmethod. DNA fingerprinting was carried out using EcoRI restriction enzyme. RESULTS:  DNA could be extracted from residues of saliva, DNA fingerprinting done with the isolated DNA was able to match with those of individuals. Chelex method was found to be more efficient than the Phenol-chloroform method KEY WORDS: Betel Quid, Chelex method, DNA,DNA fingerprinting,Phenol chloroform method Introduction DNA fingerprinting  has ascertained an increasingly imperative role towards decision making in judiciary. DNA tests have helped convict suspects, to exonerate suspects or overturned previous convictions. Scientific evidences such as fingerprints, blood, semen, shreds of clothing, hair, weapons, tire tracks, and other physical evidence at the crime scene can be a more riveting to a tribunal than the testimony of an eyewitness. DNA is more suitable because DNA remains scathe lessin challenging environments where such evidence is found. The DNA molecule holds an impressive dependability to withstand time. 1 DNA profiling compares the DNA fragment lengths and patterns. The isolated DNA from the samples is fragmented using a restriction enzyme. Then the length of the resulting fragments is determined by electrophoresis and comparedby a visual interpretation of the pattern of DNA bands. 2 DNA can be sourced from freshblood, fresh or dried human buccalswabs, soft tissue, saliva and salivary stains. Optimizing the methodology in DNA extraction from various sources have been tried by many studies. Minute quantities of saliva allows establishing DNAprofile. 3DNA has been proven to be isolated from cell samples from objects that was in contact with the body and from sources like chewing gums, cigarettes, bite marks in foods, among others. Restriction fragment length polymorphism (RFLP) analysis provides details of the DNA which is referred as a DNA fingerprint. As DNA is unique to every individual, analyzing the sequence helps in identification of specific patterns of each individual. DNA profile is considered as valid evidence in the court of law for paternity disputes and human identification. Standardization of DNA extraction technique will improve the reliability and speed up sample processing time. 4-6 Limited availability of biological samples in a crime scenechallenges the procedure of extraction , characterization and analysis of DNA. Furthermore, difficulty arises in retrieving DNA from stains and degraded samples which provide contaminated or poor qualityDNA. Hence, purification of DNA from samples is still a significant step in obtaining useful genotypes. Notwithstanding, tremendous advances have been made in the recent times in DNA testing. 7 Chewed betel quid (BQ) stains are encountered frequently on crime scenes in Southeast Asian countries. Though the quid presents as an important biological evidence, the forensic analysis using betel quid as an evidence has been impeded due to difficulty in extraction of human DNA . Hence, constituting a definite method for extracting DNA from chewed Betel quid residues is of paramount importance. 8 Saliva found on victims of several violent crimes is a potential source of DNA. They can be recovered from bite marks, cigarette butts, betel quid, postage stamps, envelopes and other objects. However , salivary stains usually dry up easily becoming invisible, making recognition and collection difficult. Among the various biological sources available, salivary analysis have great discriminatory power and can be incorporated into a criminal investigation . Improvisation of DNA extraction procedures will improve its reliability and also help to expedite the process. The present study aims to isolate DNA from blood, saliva (under different conditions) by phenol chloroform method and chelex method and to find the efficacy of these methods in extraction of DNA from traces of saliva. 9,10 ISOLATION OF DNAFROM BLOOD AND SALIVA BY PHENOL CHLOROFORM METHOD : The DNA was extracted with an equal volume of phenol: chloroform: isoamyl alcohol. This mixture was centrifuged at 10000rpm for 5 minutes. The aqueous phase was collected and extracted with chloroform: isoamyl alcohol mixture and centrifuged at 10000rpm for 5 minutes. The supernatant was transferred to a new microfuge tube and 0. 6 volume of isopropanol was added. The spongy white precipitate was transferred to a microfuge tube and added equal volume of ethanol was added. Then it was centrifuged at 10000rpm at room temperature for 10 minutes. The supernatant was drained and to the pellet 100 µL of TE buffer was added stored at 4 °C. ISOLATION OF DNAFROM BLOOD AND SALIVA BY CHELEX METHOD: 0. 5 ml of whole blood was collected in 2 ml tube and the cells are harvested by centrifugation at 3000 rpm for 3 min. at 4 °C. The supernatant was discarded. 0. 8 ml TBP buffer was added to the collection tube, vortexed gently, then centrifuged at 3000 rpm for 3 minutes, supernatant was discarded. The next stepwas continued if the blood pellet looks mauve 0. 5 ml of TBM buffer was added to the tube, and vortexed followed by addition of 3  µLof proteinase K and incubated at 55 °C for 30 minutes. The sample was centrifuged for 2 minutes at 5000 rpm and the supernatant saved to 2 ml tube and then added 260  µL of absolute ethanol. The mixture was applied to EZ-10 column, centrifuged at 8000 rpm for 1 minute; discarded the flow in the collection tube. 500  µL of wash solution was added and centrifuged at 8000 rpm for 1 minute. This step was repeated spin at 8000 rpm for an additional minute to remove residual amount of wash solution. The column was placed into a clean 1. 5 ml microfuge tube and 30  µL of elution buffer was added into the center part of membrane . The tube was incubated at 50 °C for 2 minutes centrifuged at 10,000 rpm for 1 minute to elute the DNA from the column The standards and samples were removed from the freezer and thawed. In a separate sterile 1. 5 ml microfuge tube for each standard/sample, 10  µl of DNA was mixed with 990  µl of D. I. water and vortexed . The solution was allowed to stand for 10 minutes to ensure the complete diffusion of DNA throughout the solution. This represents a 1:100 dilution of the standards and the DNA samples. B. DNAquantification The DNA sample was briefly vortexed and the solution wastransfered to the cuvette of the spectrophotometer with care not to create bubbles. The cuvette is inserted into the spec ensuring the correct face of the cuvette is in line the light beam. . An absorbance reading appears on the screen . Reading is continued until all standards and samples have been quantified. The concentration of DNA in the sample is determined according to the conversion factor (A260 of 1. 0 = 50  µg ml-1 DNA). The concentration of DNA in the sample can be read as  µg/mL using the conversion factor and dilution factor . RESTRICTION DIGESTION: Restriction enzyme buffer was vortexed before pipetting to ensure that it was well-mixed and was added to the tube . Appropriate amount of DNA to be cut wasvortexed before pipetting to ensure that it was well-mixed and was added to the tube. After vortexingthe enzyme to ensure that it was well-mixed 1 ÃŽ ¼L of enzyme EcoRIwas added. The mixture is placed in thermal cycler (Eppendorf) for2-3 hour incubation at 37 °C . To heat inactivate the enzyme the mixture is maintained at 80 °C for 20 min. The mixture is kept at 4 °C until the reaction mixture is out of the thermal cycler. Agarose Gel Electrophoresis Protocol Preparation of the agarose gel 1. 25 g Agarose powder was taken in 500 ml flask and 125 ml of TAE Buffer was added to it. The mixture is melted in hot water bath till a clear solution forms. The solution is allowed to cool to a temperature of 50-55 °C by periodic swirling to achieve even cooling. To it ethidium bromide solution was added. The ends of the casting tray are sealed with two layers of tape. The combs are placed in the gel casting tray. The melted agarose solution was poured into the casting tray and allowed to cool until it is solid. The comb and the tape are removed carefully. The gel is placed in the electrophoresis chamber. 2-3 mm of TAEBuffer is added over the gel. Loading the gel 6 à ¯Ã‚ Ã‚ ­l of 6X Sample Loading Buffer is added to each DNA sample containing tubes. 20 à ¯Ã‚ Ã‚ ­l of each sample is pipetted into separate wells in the gel. 10 à ¯Ã‚ Ã‚ ­l of the DNA ladder standard is pippeted into one well of each row on the gel. Running the gel The lid is place on the gel box, the electrode wires are connected to the power supply. The power supply is turned on to about 100 volts. To ensure the correct direction of the current, the movement of the blue loading dye is checked. The power supply is continued till the blue dye approaches the end of the gel. The wires are disconnected from the power supply. The lid is removed from the electrophoresis chamber. Using gloves, gel is carefully removed and observed in a transilluminator for the DNA bands. RESULTS: Isolation of DNA was done from blood ,fresh saliva, saliva stored at -20 °C, saliva stored at 37 °C for 24hrs ,buccal swab and betel quid by both the phenol-chloroform method and the chelex method. . Gel electrophoresis of the isolated genomic DNA was carried out on 0. 8% agarose gel. (figure 1) After restriction digestion electrophoresis gel is prepared to run and to identify the number of bands. DNA samplesobtained from blood were labelled as Aband subsequently as Bb,Cb, DbEbas shown in table 2. DNA obtained from fresh saliva were labelled as As,Bs, Cs, Ds,Es. DNA obtained from saliva stored at -20 degree were labeled as AfsBfsCfsDfs. Efs. DNA obtained from saliva stored at room temperature were labelled as Ads,BdsCdsDdsEds DNA obtained from bloodof 5 individuals was made to run in the well marked 1 to 5 in a uniform manner ie DNA obtained from the first individual named as Ab, was made to run in well No. 1 . DNA obtained from second individual named as Bb was made to run in well No. 2 DNA obtained from third individual named as Cb was made to run in well No . 3. DNA obtained from fourth individual named as Db was made to run in as well No- 4. DNA obtained from Fifth individual namedEbwas made to run in well No. 5. (table 2) But while running DNA obtained from saliva of different sources the order was changed randomly. For example DNA isolated from fresh saliva for the first individual (As) instead of being run in the first well ie well No -6 was made to run in the third well( well no 8) and DNA isolated from saliva stored at -20 degree for the first individual(Afs) instead of being run in the first well ie well No-11was made to run in the third well (well No. 13)and DNA isolated from saliva stored at room temperature for the first individual(Ads) instead of being run in the first well ie well No-16 was made to run in the fifth well (well No. 20). Likewise DNA isolated from different sources of saliva of different individuals made to run in different wells and the number of bands produced is identified . From the figure 1 it could be identified that the well number 1,8 ,13,20 corresponding to DNA isolated from the first individual from various sources named Ab AsAfs Ads identified by the yellow arrow has uniformly three bands. For the well number 2,7,14,19 corresponding to DNA isolated from the second individual from various sources named BbBsBfsBds identified by the blue arrow has uniformly 6 bands . various DNA isolated from the fifth individual from various sources namedEbEsEfsEds identified by the green arrow has uniformly 4 bands . From the above figure itcould be identified that the well number 1,10 corresponding to DNA isolated from different source for the first individual named Ab,AbS,identified by the yellow arrow has uniformly four bands. For the well number 2 and 6 corresponding to DNA isolated from second individual from blood and buccal swab named BbBbSl identified by the blue arrow has uniformly 6 bands . For the well number 3 and 7corresponding to DNA isolated from third individual from blood and buccal swab namedCbCbS identified by the red arrow has uniformly 5 bands . For the well number 4and 8 corresponding to DNA isolated from fourth individual from blood and buccal swab named DbDbs,identified by the aqua arrow has uniformly 7 bands. For the well number 5and 9 corresponding to DNA isolated from fifth individual from blood and buccal swab named EbEbsE identified by the green arrow has uniformly 8 bands . This shows that DNA obtained from an individual from blood and buccal swab produce uniform banding pattern. This shows that DNA obtained from an individual from various source produce uniform banding pattern . Identification of individual from traces of saliva which could be used for forensic application -Extraction of DNA from Buccal swab. Restriction digestion with Ecor-1 from extracted DNA obtained from above mentioned source has been done for identifying individuals. Blood was used as a control and compared with DNA bands from buccal swab. A total of 10 wells were created. DNA obtained from blood wer e labeled as Ab, Bb,Cb, Db,Ebas shown in tab 3. DNA obtained from Buccal swab were labeled as Abs,Bbs, Cbs, Dbs, Ebs. DNA obtained from blood from 5 individuals was made to run in the well marked 1 to 5 in a uniform manner. But while running DNA obtained from buccal swab the order was changed randomly. For example DNA isolated from buccal swab for the first individual (Abs) instead of being run in the first well ie well No -6 was made to run in the fifth well( well no 10). Likewise DNA isolated from buccal swab of different individuals was made to run in different wells and the number of bands produced is identified Different methods of DNA extraction is been followed in that, most widely used is phenol chloroform method . Many new methods of DNA extraction have been tried. The chelex method is one among then . To know the efficacy of the chelex method it was compared with that of phenol chloroform method. Of the two methods studied the chelex method proved to be more easy to handle and less time consuming in addition to yieds higher amount of DNA and is proved by quantification with U. V spectrometer as shown in fig. 2. DISCUSSION: Forensic odontology is a branch of forensics which analyses stains and organic liquids from the oral cavity or its contents, bite mark comparison, investigation of trauma and oral injuries such as personal injury cases, and dental malpractice. The fundamental requirement of a criminal investigation is that the victim and aggressor should be positively identified. Forensic dentistry aids in the forensic process by comparing the deceaseds dentition with that of previous dental records or by facilitating to shape the profile of an individual in terms of age at the time of death, sex and phylogeny to aid in identification. 11,12 Saliva has been a potential source of identification and is usually found in bite marks, cigarette butts, betel quid, postage stamps, envelopes and other objects. The first phase of the study intended to isolate DNA from saliva (under different conditions), by phenol-chloroform method and chelex method and compare the yield with that of blood . The second objective was to find out efficacy of these methods in extraction of DNA from traces of saliva ie from Buccal swab, and from Betel quid and which could be used for forensic application. 8 The presence of residues are considerably important as biological evidences, but forensic analysis of such evidences has been hindered by failures in extraction of human DNA. Consequently, it is indispensable in forensic science to establish a reliable method for extracting DNA from samples collected at the crime site. The most important objective was whether individuals can be identifed from samples of different source and to ascertain the applicability of the restriction digestion in forensics. 13,14 Blood was taken as control, saliva was divided into 3 parameters ie from fresh saliva, from saliva stored at -20 degree for24 hr from saliva stored in room temperature for 24 hr’s were obtained . Identification of individual has been done with restriction enzyme EcoRI. . The isolated DNA was digested using the restriction enzyme EcoRI(G|AATTC)The digested DNA was run on 1% agarose gel electrophoresis and the bands produced in each individuals DNA were scored and is proved that identification of individual can also be done by DNA fingerprinting or profiling. Agarose gel electrophoresis separates DNA fragments according to their size. The most important objective was whether individuals can be identified from samples of different source and to ascertain the applicability of the restriction digestion in forensics. 16 DNA fingerprinting is a technique that is used to represent like and unlike DNA that is present in different individuals. Nucleotide sequences which show significant variation from one individual to another are taken into consideration. 17The most important objective of the study was to ascertain whether individuals can be identified from samples of different source and to ascertain the applicability of the restriction digestion in forensics and the last objective was toCompare the DNA yield from manual and kit method. To prove that DNA could be extracted from traces of saliva , Buccal swab and Beetal quid was used . DNA could be extracted from buccal swab,beetal quid and quantification was done with U. V spectrometer. Comparison of DNA isolated from all the samples collected from all the individual using two different procedures has been done and comparison of yield of different sources showed the kit method to be more effective . Use of biological evidences like saliva, buccal swab and betel quid are compromised due to the quandary in extraction of human DNA. The present study had proved to establish a reliable method for extracting DNA from samples collected from different sources of saliva and from traces of salivary stains which was comparable to bloodin proving identification. Samples collected from different sources of saliva and from traces of salivary stains can also be assessed by DNA fingerprinting or profiling which is based on the fact that DNA is unique to every individual .